Programme
Programme
The contents of the School comprise a diverse and all-encompassing programme, a mixture of lectures and laboratory practical work.
Highlights of 2023
Enhancing the FDA’s Regulatory Science Approach for the Review of PFA/IRE Devices, Ksenia Blinova
Human in vitro assay for non-clinical evaluation Pulsed Field Ablation treatments, Maura Casciola
Collateral effects of Electroporation (heating, pain & contraction, electrochemistry), Antoni Ivorra
The use of moderate and high voltage electric fields in food processing and the circular bioeconomy, James Lyng
Will pulsed field ablation change treatment of cardiac arrhythmias, Atul Verma
Preliminary programme
Electrical properties of cells and tissues in electric field
- Resting and induced transmembrane voltage
- Dielectric properties of biological tissues
- Temperature and conductivity changes in tissues
Physical chemistry of membrane electroporation
- Electrochemical membrane thermodynamics
- Molecular dynamics simulations
Electroporation in vitro and in vivo – protocols
- Electroporation of cells in vitro
- Electroporation of cells in vivo
Development of devices and electrodes
- Devices for in vitro and in vivo experimental work
- Electrodes for in vitro and in vivo
- Electroporation of cells in tissue – models of electroporation in vivo and their experimental validations
Electrochemotherapy of tumors
- Basic mechanisms of electrochemotherapy
- Preclinical studies
- Clinical use of electrochemotherapy
- Current developments and future perspectives
Application of electroporation in gene transfection
- Gene transfection in vitro
- Gene transfection in vivo
- Electroporation for gene therapy
- Electroporation for DNA vaccine
Laboratory and practical work
More details on the Labwork page...
- The influence of Mg2+ ions on gene electotransfer efficiency
- Monitoring cell membrane electropermeabilization with ratiometric fluorescence dye FURA-2AM
- Electropermeabilization detection with propidium iodide in plated cells
- Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS
- Electrotransformation of Escherichia coli with plasmid DNA
- E. coli inactivation in a continuous flow system
- Electroporation of planar lipid bilayers
- Analysis of electric field orentations on gene electrotransfer visualization at the membrane level
- Treatment planning for electrochemotherapy and irreversible electroporation: optimization of voltage and electrode position
- Numerical Modeling of Thermal Effects during Irreversible Electroporation Treatments
- Molecular dynamics simulations of membrane electroporation
- Electroporation of cells and tissues – interactive e-learning
- Measurement of electroporation pules with osciloscope; voltage and current probes
- Development of pulse power generators for electroporation