Practical work

You will be able to choose practical work sessions only after you have paid the registration fee and have passed the laboratory safety test. Your chosen practical work preferences will be taken into account, until the places for practical work sessions are filled. Sessions with a limited number of participants will be filled on a first-come-first-served basis. Please be sure to mark all practical work sessions by priority, as practical work sessions have limited number of participants, therefore it’s possible that your second or third choice will be considered instead of your first one.

Looking for the practical work schedule? See below!

Laboratory safety test

You will be able to take laboratory safety test and select practical work sessions in e-learning platform after registration. You should receive an e-mail with instructions how to access the e-learning platform.

S1 – Laboratory safety

S2 – Electroporation hardware safety

List of laboratory exercises

Below you can find a list of this year practical work sessions. Due to the hybrid nature of 2022 edition of EBTT, we are offering on-site practical work sessions as well as interactive online training sessions for those who are participating EBTT online.

Laboratory exercises

Labwork On-site On-line
  L1 – The influence of Mg2+ ions on gene electotransfer efficiency    
  L2 – Monitoring cell membrane electroporation with ratiometric fluorescent dye Fura-2AM    
  L3 – Visualisation of local ablation zone distribution between two needle electrodes    
  L4 – Effect of short high-frequency bipolar pulses on plasma membrane permeabilization    
  L5 – Electroporation of planar lipid bilayers    
  L6 – Eradication of antibiotic-resistant E. coli by the combination of antibiotics and electroporation in a continuous mode    
  L7 – Analysis of electric field orientations on gene electrotransfer – visualization at the membrane level    
  L8 – Comparison of flow cytometry and spectrofluorometric measurements in cell permeabilization experiments    
  L9 – Monitoring of electric field distribution in biological tissue by means of magnetic resonance electrical impedance tomography    
  L10 – Measurements of the induced transmembrane voltage with fluorescent dye di-8-ANEPPS    
  L11 – Analysis of electric field orientations on gene electrotransfer efficiency    
  L12 – Monitoring cell membrane depolarization due to electroporation using fluorescent plasma membrane potential indicator    
  L13 – Impedance and texture analysis techniques for detecting and characterising electroporation in plant tissues    
  L14 – Modelling, visualising, and tracking pH front formation during pulse delivery to agarose-based tissue phantoms    
  L15 – Simultaneous measurement of sarcomere shortening and calcium transients in primary rat cardiomyocytes exposed to electroporation electrical pulses